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验证数据展示

  • WB analysis of HEK-293 using 11439-1-AP

    WB analysis of HEK-293 using 11439-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11439-1-AP (GRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 11439-1-AP

    IP experiment of HEK-293 using 11439-1-AP

    IP result of anti-GRK6 (IP:11439-1-AP, 4ug; Detection:11439-1-AP 1:500) with HEK-293 cells lysate 1120ug.

  • IHC staining of human renal cell carcinoma using 11439-1-AP

    IHC staining of human renal cell carcinoma using 11439-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11439-1-AP (GRK6 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 11439-1-AP

    IHC staining of human renal cell carcinoma using 11439-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11439-1-AP (GRK6 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

GRK6 Polyclonal antibody

GRK6 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11439-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

GPRK6, GRK6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11439-1-AP targets GRK6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 GRK6 fusion protein Ag1992 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor kinase 6
别名 GPRK6, GRK6
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC017272
基因名称 GRK6
Gene ID (NCBI) 2870
RRIDAB_2248070
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43250
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRK6 antibody 11439-1-APDownload protocol
IHC protocol for GRK6 antibody 11439-1-APDownload protocol
IP protocol for GRK6 antibody 11439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.

Authors - Fan Yang
  • KD Validated
View Article
humanWB,IHC

Front Oncol

GRK6 Depletion Induces HIF Activity in Lung Adenocarcinoma.

Authors - Sumei Yao
View Article