- Featured Product
- KD/KO Validated
GRLF1 Polyclonal antibody
GRLF1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells |
Positive IHC detected in | human colon tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26789-1-AP targets GRLF1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | GRLF1 fusion protein Ag25353 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glucocorticoid receptor DNA binding factor 1 |
Synonyms | ARHGAP35, GRF 1, GRF1, GRLF1, KIAA1722, P190 A, P190A, p190RhoGAP, Rho GAP p190A |
Observed Molecular Weight | 172 kDa |
GenBank Accession Number | BC150257 |
Gene Symbol | GRLF1 |
Gene ID (NCBI) | 2909 |
RRID | AB_2880636 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NRY4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GRLF1 antibody 26789-1-AP | Download protocol |
IHC protocol for GRLF1 antibody 26789-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.
| ||
Oncogene Ubiquitin ligase TRIM65 promotes colorectal cancer metastasis by targeting ARHGAP35 for protein degradation. | ||
Front Cell Dev Biol Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration. | ||
Cell Death Discov The m6A demethylases FTO and ALKBH5 aggravate the malignant progression of nasopharyngeal carcinoma by coregulating ARHGAP35 |