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验证数据展示

  • WB analysis using 12720-1-AP

    WB analysis using 12720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 12720-1-AP

    IP experiment of MCF-7 using 12720-1-AP

    IP result of anti-GRPEL1 (IP:12720-1-AP, 4ug; Detection:12720-1-AP 1:10000) with MCF-7 cells lysate 1000 ug.

  • IHC staining of human bowen disease using 12720-1-AP

    IHC staining of human bowen disease using 12720-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12720-1-AP (GRPEL1 antibody) at dilution of 1:2400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human bowen disease using 12720-1-AP

    IHC staining of human bowen disease using 12720-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12720-1-AP (GRPEL1 antibody) at dilution of 1:2400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 12720-1-AP

    IF Staining of HepG2 using 12720-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRPEL1 antibody (12720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 12720-1-AP

    IF Staining of HepG2 using 12720-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRPEL1 antibody (12720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

GRPEL1 Polyclonal antibody

GRPEL1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12720-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Mt-GrpE#1, Mt GrpE#1, HMGE, GREPEL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12720-1-AP targets GRPEL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 GRPEL1 fusion protein Ag3415 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GrpE-like 1, mitochondrial (E. coli)
别名 Mt-GrpE#1, Mt GrpE#1, HMGE, GREPEL1
计算分子量 217 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC024242
基因名称 GRPEL1
Gene ID (NCBI) 80273
RRIDAB_2114331
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAV7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRPEL1 antibody 12720-1-APDownload protocol
IHC protocol for GRPEL1 antibody 12720-1-APDownload protocol
IF protocol for GRPEL1 antibody 12720-1-APDownload protocol
IP protocol for GRPEL1 antibody 12720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin
View Article
humanWB

Nat Commun

Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy

Authors - Jonas Benjamin Michaelis
View Article
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen
View Article