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BET1L Polyclonal antibody

BET1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14163-1-AP

Print datasheet

Synonyms

Vesicle transport protein GOS15, GS15, GOS-15, Golgi SNARE with a size of 15 kDa, BET1-like protein

验证数据展示

  • WB analysis using 14163-1-AP

    WB analysis using 14163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14163-1-AP (BET1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 14163-1-AP

    IHC staining of human cervical cancer using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14163-1-AP (BET1L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 14163-1-AP

    IHC staining of human cervical cancer using 14163-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14163-1-AP (BET1L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14163-1-AP targets BET1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BET1L fusion protein Ag5351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name blocked early in transport 1 homolog (S. cerevisiae)-like
Synonyms Vesicle transport protein GOS15, GS15, GOS-15, Golgi SNARE with a size of 15 kDa, BET1-like protein
Calculated Molecular Weight 102 aa, 11 kDa
Observed Molecular Weight13-15 kDa
GenBank Accession NumberBC032779
Gene Symbol BET1L
Gene ID (NCBI) 51272
RRIDAB_2064737
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BET1L antibody 14163-1-APDownload protocol
IHC protocol for BET1L antibody 14163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols