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验证数据展示

  • WB analysis of NIH/3T3 using 16106-1-AP

    WB analysis of NIH/3T3 using 16106-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16106-1-AP

    IF Staining of HeLa using 16106-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16106-1-AP (GS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 16106-1-AP

    IHC staining of human stomach cancer using 16106-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach cancer using 16106-1-AP

    IHC staining of human stomach cancer using 16106-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 40x lens).

GS28 Polyclonal antibody

GS28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16106-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, human liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16106-1-AP targets GS28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GS28 fusion protein Ag9021 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi SNAP receptor complex member 1
别名 28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28
计算分子量28.6 kDa
观测分子量 28 kDa
GenBank蛋白编号BC012620
基因名称 GS28
Gene ID (NCBI) 9527
RRIDAB_2294912
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95249
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

实验方案

Product Specific Protocols
WB protocol for GS28 antibody 16106-1-APDownload protocol
IHC protocol for GS28 antibody 16106-1-APDownload protocol
IF protocol for GS28 antibody 16106-1-APDownload protocol
FC protocol for GS28 antibody 16106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols