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GSDMB Monoclonal antibody

GSDMB Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67527-1-Ig

Print datasheet

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

验证数据展示

  • WB analysis of MCF-7 using 67527-1-Ig

    WB analysis of MCF-7 using 67527-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67527-1-Ig

    IF Staining of HepG2 using 67527-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSDMB antibody (67527-1-Ig, Clone: 2F12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inMCF-7 cells, COLO 320 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67527-1-Ig targets GSDMB in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen GSDMB fusion protein Ag28332 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name gasdermin B
Synonyms gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521
Calculated Molecular Weight 411 aa, 47 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC025682
Gene Symbol GSDMB
Gene ID (NCBI) 55876
RRIDAB_2882746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TAX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSDMB antibody 67527-1-IgDownload protocol
IF protocol for GSDMB antibody 67527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Arch Biochem Biophys

GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis

Authors - Xiaoping Xie
View Article