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验证数据展示

  • WB analysis using 30469-1-AP

    WB analysis using 30469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30469-1-AP (GSDMC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of Human bowens disease using 30469-1-AP

    IHC staining of Human bowens disease using 30469-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 30469-1-AP (GSDMC antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GSDMC Polyclonal antibody

GSDMC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30469-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Gasdermin-C, MLZE, gasdermin C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, HCT 116 cells, A375 cells
Positive IHC detected inHuman bowens disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30469-1-AP targets GSDMC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen GSDMC fusion protein Ag33319 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gasdermin C
Synonyms Gasdermin-C, MLZE, gasdermin C
Calculated Molecular Weight58kd
Observed Molecular Weight58 kDa
GenBank Accession NumberNM_031415
Gene Symbol GSDMC
Gene ID (NCBI) 56169
RRIDAB_3086329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gasdermin (GSDM) superfamily consists of Gasdermin family genes (GSDMA, GSDMB, GSDMC and GSDMD in humans) and Gasdermin-related genes (DFNA5, DFNB59 in humans). GSDM family members were shown to be detected in the epithelium of various tissue types in a highly tissue-specific manner and suggested to have differentiation-status specific roles. Gasdermin C (GSDMC) was known as melanoma-derived leucine zipper-containing extranuclear factor (MLZE). Several studies have suggested that GSDMC may be involved in the course of tumorigenesis such as acquisition of metastatic potential in melanoma cells or promotion of cell proliferation in colorectal carcinogenesis and that GSDMC may function as an oncogene. (PMID: 29428815)

实验方案

Product Specific Protocols
WB protocol for GSDMC antibody 30469-1-APDownload protocol
IHC protocol for GSDMC antibody 30469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols