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GSG1L Polyclonal antibody

GSG1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17328-1-AP

Print datasheet

Synonyms

GSG1 like, GSG1 like protein, GSG1L, PRO19651

验证数据展示

  • WB analysis of U-251 using 17328-1-AP

    WB analysis of U-251 using 17328-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 17328-1-AP

    WB analysis using 17328-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17328-1-AP (GSG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 17328-1-AP

    IHC staining of human tonsillitis using 17328-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 17328-1-AP

    IHC staining of human tonsillitis using 17328-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17328-1-AP (GSG1L Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inRaji cells, U-251 cells, SGC-7901 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17328-1-AP targets GSG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen GSG1L fusion protein Ag11307 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GSG1-like
Synonyms GSG1 like, GSG1 like protein, GSG1L, PRO19651
Calculated Molecular Weight 176 aa, 21 kDa
Observed Molecular Weight 36-43 kDa
GenBank Accession NumberBC016460
Gene Symbol GSG1L
Gene ID (NCBI) 146395
RRIDAB_2878384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSG1L is a component of the inner core of AMPAR complex. It modifies AMPA receptor (AMPAR) gating. GSG1L is mainly present on B lymphocytes. This antibody can recognize all the 4 isoforms of GSG1L. GSG1L was detected the isoform of 43 kDa in rat brain (PMID: 22813734).

实验方案

Product Specific Protocols
WB protocol for GSG1L antibody 17328-1-APDownload protocol
IHC protocol for GSG1L antibody 17328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rat

Nat Commun

GSG1L-containing AMPA receptor complexes are defined by their spatiotemporal expression, native interactome and allosteric sites

Authors - Amanda M Perozzo
View Article