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GSK3B Monoclonal antibody, PBS Only

GSK3B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E9E4

Cat no : 67329-1-PBS

Print datasheet

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

验证数据展示

  • WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    IHC staining of human breast cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSK3B antibody included
  • IF Staining of HeLa using 67329-1-Ig (same clone as 67329-1-PBS)

    IF Staining of HeLa using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSK3B antibody (67329-1-Ig, Clone: 2E9E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

产品信息

67329-1-PBS targets GSK3B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen GSK3B fusion protein Ag17320 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycogen synthase kinase 3 beta
Synonyms GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1
Calculated Molecular Weight 433 aa, 48 kDa
Observed Molecular Weight 46-48 kDa
GenBank Accession NumberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2882588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49841
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .