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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    IHC staining of human breast cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSK3B antibody included
  • IF Staining of HeLa using 67329-1-Ig (same clone as 67329-1-PBS)

    IF Staining of HeLa using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSK3B antibody (67329-1-Ig, Clone: 2E9E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

GSK3B Monoclonal antibody, PBS Only

GSK3B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67329-1-PBS

产品说明书

CloneNo. 2E9E4

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

产品信息

67329-1-PBS targets GSK3B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 GSK3B fusion protein Ag17320 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycogen synthase kinase 3 beta
别名 GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1
计算分子量 433 aa, 48 kDa
观测分子量 46-48 kDa
GenBank蛋白编号BC000251
基因名称 GSK3B
Gene ID (NCBI) 2932
RRIDAB_2882588
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49841
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .