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GSK3B Recombinant antibody, PBS Only

GSK3B Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4N21

Cat no : 82061-1-PBS

Print datasheet

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

验证数据展示

  • WB analysis using 82061-1-RR (same clone as 82061-1-PBS)

    WB analysis using 82061-1-RR (same clone as 82061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82061-1-RR (GSK3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82061-1-RR (same clone as 82061-1-PBS)

    IP experiment of HeLa using 82061-1-RR (same clone as 82061-1-PBS)

    IP result of anti-GSK3B (IP:82061-1-RR, 4ug; Detection:82061-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

    IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSK3B antibody included
  • Affinity and Kinetic Characterization of 82061-1-RR

    Affinity and Kinetic Characterization of 82061-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82061-1-RR against Human GSK3B were performed. The affinity constant is 7.36 pM.

Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

产品信息

82061-1-PBS targets GSK3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GSK3B fusion protein Ag17320 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycogen synthase kinase 3 beta
Synonyms GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1
Calculated Molecular Weight 433 aa, 48 kDa
Observed Molecular Weight~45 kDa
GenBank Accession NumberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49841
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.