• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Monoclonal antibody, PBS Only

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E6E2

Cat no : 66193-1-PBS

Print datasheet

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

验证数据展示

  • WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

产品信息

66193-1-PBS targets GSNOR,ADH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen GSNOR,ADH5 fusion protein Ag9511 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Synonyms ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5
Calculated Molecular Weight 374 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC014665
Gene Symbol ADH5
Gene ID (NCBI) 128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11766
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).