验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lung cancer tissue, human liver tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14475-1-AP targets GSTA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GSTA1 fusion protein Ag5849 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutathione S-transferase alpha 1 |
| 别名 | Glutathione S transferase A1, EC:5.3.3.-, EC:2.5.1.18, EC:1.11.1.-, Androst-5-ene-3,17-dione isomerase |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC053578 |
| 基因名称 | GSTA1 |
| Gene ID (NCBI) | 2938 |
| RRID | AB_2232810 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08263 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTA1 antibody 14475-1-AP | Download protocol |
| IHC protocol for GSTA1 antibody 14475-1-AP | Download protocol |
| IF protocol for GSTA1 antibody 14475-1-AP | Download protocol |
| IP protocol for GSTA1 antibody 14475-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions | ||
Sci Adv Treatment with siRNAs is commonly associated with GPX4 up-regulation and target knockdown-independent sensitization to ferroptosis | ||
Cell Death Dis Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity. | ||
Food Chem Toxicol l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway. |
