• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GSTA1 Monoclonal antibody

GSTA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1F4

Cat No : 66624-1-Ig

Print datasheet

Synonyms

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

验证数据展示

  • WB analysis of pig liver using 66624-1-Ig

    WB analysis of pig liver using 66624-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 66624-1-Ig

    WB analysis of mouse liver using 66624-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 66624-1-Ig

    WB analysis of rat liver using 66624-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66624-1-Ig

    IHC staining of human kidney using 66624-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human testis using 66624-1-Ig

    WB analysis of human testis using 66624-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66624-1-Ig

    IF Staining of HepG2 using 66624-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66624-1-Ig (GSTA1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig liver tissue, human liver tissue, rat kidney tissue, mouse hepatocytes, human testis tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66624-1-Ig targets GSTA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat, pig
Cited Reactivitypig
Immunogen GSTA1 fusion protein Ag6084 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase alpha 1
Synonyms Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC053578
Gene Symbol GSTA1
Gene ID (NCBI) 2938
RRIDAB_2881984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSTA1 antibody 66624-1-IgDownload protocol
IHC protocol for GSTA1 antibody 66624-1-IgDownload protocol
IF protocol for GSTA1 antibody 66624-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

J Anim Physiol Anim Nutr (Berl)

Tolerable upper intake level of iron damages the liver of weaned piglets.

Authors - Haoxuan Ding
View Article