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验证数据展示

  • IHC staining of human stomach cancer using 55474-1-AP

    IHC staining of human stomach cancer using 55474-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55474-1-AP (GSTM2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 55474-1-AP

    IHC staining of human stomach cancer using 55474-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55474-1-AP (GSTM2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 55474-1-AP

    WB analysis using 55474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55474-1-AP (GSTM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55474-1-AP

    IF Staining of HeLa using 55474-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM2 antibody (55474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GSTM2 Polyclonal antibody

GSTM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55474-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

GTHMUS, GSTM2-2, GSTM, GST4, GST class mu 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55474-1-AP targets GSTM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase mu 2 (muscle)
别名 GTHMUS, GSTM2-2, GSTM, GST4, GST class mu 2
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号NM_000848
基因名称 GSTM2
Gene ID (NCBI) 2946
RRIDAB_3086453
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28161
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutathione S-Transferase Mu 2 (GSTM2) is a phase II metabolizing enzyme that conjugates glutathione to electrophilic compounds. GSTM2 is closely related to cancer progression, and there have been several publications regarding the function of GSTM2 in drug resistance (PMID: 36038661). Western blot analysis detected GSTM2 at an apparent molecular mass of 26 kDa.

实验方案

Product Specific Protocols
WB protocol for GSTM2 antibody 55474-1-APDownload protocol
IHC protocol for GSTM2 antibody 55474-1-APDownload protocol
IF protocol for GSTM2 antibody 55474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols