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验证数据展示

  • IHC staining of human stomach cancer using 55474-1-AP

    IHC staining of human stomach cancer using 55474-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55474-1-AP (GSTM2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 55474-1-AP

    IHC staining of human stomach cancer using 55474-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55474-1-AP (GSTM2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 55474-1-AP

    WB analysis using 55474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55474-1-AP (GSTM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55474-1-AP

    IF Staining of HeLa using 55474-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM2 antibody (55474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GSTM2 Polyclonal antibody

GSTM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55474-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GTHMUS, GSTM2-2, GSTM, GST4, GST class mu 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55474-1-AP targets GSTM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione S-transferase mu 2 (muscle)
Synonyms GTHMUS, GSTM2-2, GSTM, GST4, GST class mu 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberNM_000848
Gene Symbol GSTM2
Gene ID (NCBI) 2946
RRIDAB_3086453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutathione S-Transferase Mu 2 (GSTM2) is a phase II metabolizing enzyme that conjugates glutathione to electrophilic compounds. GSTM2 is closely related to cancer progression, and there have been several publications regarding the function of GSTM2 in drug resistance (PMID: 36038661). Western blot analysis detected GSTM2 at an apparent molecular mass of 26 kDa.

实验方案

Product Specific Protocols
WB protocol for GSTM2 antibody 55474-1-APDownload protocol
IHC protocol for GSTM2 antibody 55474-1-APDownload protocol
IF protocol for GSTM2 antibody 55474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols