验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HCT 116 cells, Caco-2 cells, SCaBER cells, MCF-10A cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy GSTP1 Ready-To-Use IHC Kit. GSTP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66715-1-Ig targets GSTP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GSTP1 fusion protein Ag8880 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutathione S-transferase pi 1 |
| 别名 | 2D4A6, EC:2.5.1.18, FAEES3, Glutathione S-transferase P, GST3 |
| 计算分子量 | 210 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC010915 |
| 基因名称 | GSTP1 |
| Gene ID (NCBI) | 2950 |
| RRID | AB_2882066 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09211 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| IHC protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| IF protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| IP protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med Rep Targeted reversal of multidrug resistance in ovarian cancer cells using exosome‑encapsulated tetramethylpyrazine | ||
Phytomedicine Natural small-molecules reverse Xeroderma Pigmentosum Complementation Group C (XPC) deficient-mediated drug-resistance in renal cell carcinoma | ||
Eur J Pharmacol N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis | ||
Bioorg Chem Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1 | ||
Free Radic Biol Med Ginsenoside Rg1 protects cardiac mitochondrial function via targeting GSTP1 to block S-glutathionylation of optic atrophy 1 |
