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  • KD/KO Validated

GSTP1 Monoclonal antibody

GSTP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2D4A6

Cat no : 66715-1-Ig

Print datasheet

Synonyms

GST3/GST pi, GST3, Glutathione S-transferase P, FAEES3, EC:2.5.1.18



经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GSTP1 Ready-To-Use IHC Kit. GSTP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66715-1-Ig targets GSTP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Immunogen GSTP1 fusion protein Ag8880 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase pi 1
Synonyms GST3/GST pi, GST3, Glutathione S-transferase P, FAEES3, EC:2.5.1.18
Calculated Molecular Weight 210 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC010915
Gene Symbol GSTP1
Gene ID (NCBI) 2950
RRIDAB_2882066
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09211
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSTP1 antibody 66715-1-IgDownload protocol
IHC protocol for GSTP1 antibody 66715-1-IgDownload protocol
IF protocol for GSTP1 antibody 66715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

PMID: 32535103

Authors - Qi Feng
  • KD Validated
humanWB

Mol Med Rep

Targeted reversal of multidrug resistance in ovarian cancer cells using exosome‑encapsulated tetramethylpyrazine

Authors - Chenge Zhao
humanIF

Phytomedicine

Natural small-molecules reverse Xeroderma Pigmentosum Complementation Group C (XPC) deficient-mediated drug-resistance in renal cell carcinoma

Authors - Ruihong Chen
mouseCoIP

Eur J Pharmacol

N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis

Authors - Wan-Li Duan
humanWB

Bioorg Chem

Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1

Authors - Qiu-Lin Yan
ratWB

Free Radic Biol Med

Ginsenoside Rg1 protects cardiac mitochondrial function via targeting GSTP1 to block S-glutathionylation of optic atrophy 1

Authors - Huimin Zhu