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验证数据展示

  • WB analysis of rat liver using 15063-1-AP

    WB analysis of rat liver using 15063-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15063-1-AP (GSTT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15063-1-AP

    IHC staining of human prostate cancer using 15063-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15063-1-AP (GSTT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSTT2 antibody included
  • IHC staining of human prostate cancer using 15063-1-AP

    IHC staining of human prostate cancer using 15063-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15063-1-AP (GSTT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSTT2 antibody included
  • IF Staining of L02 using 15063-1-AP

    IF Staining of L02 using 15063-1-AP

    Immunofluorescent analysis of L02 cells using 15063-1-AP (GSTT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GSTT2 Polyclonal antibody

GSTT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15063-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GSTT2B, GST class-theta-2, GST class theta 2, Glutathione S-transferase theta-2, EC:2.5.1.18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue, L02 cells, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy GSTT2 Ready-To-Use IHC Kit. GSTT2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15063-1-AP targets GSTT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 GSTT2 fusion protein Ag7066 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase theta 2
别名 GSTT2B, GST class-theta-2, GST class theta 2, Glutathione S-transferase theta-2, EC:2.5.1.18
计算分子量 27 kDa
观测分子量 28 kDa
GenBank蛋白编号BC002415
基因名称 GSTT2
Gene ID (NCBI) 2953
RRIDAB_2878106
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0CG29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSTT2 antibody 15063-1-APDownload protocol
IHC protocol for GSTT2 antibody 15063-1-APDownload protocol
IF protocol for GSTT2 antibody 15063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bioorg Chem

Two resveratrol analogs, pinosylvin and 4,4'-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway.

Authors - Cheng-Niu Wang
View Article