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  • Featured Product
  • KD/KO Validated

GSTT2B Polyclonal antibody

GSTT2B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17622-1-AP

Print datasheet

Synonyms

GSTT2, GST class-theta-2, GST class theta 2, Glutathione S-transferase theta-2B, Glutathione S-transferase theta-2

验证数据展示

  • WB analysis using 17622-1-AP

    WB analysis using 17622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17622-1-AP (GSTT2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17622-1-AP

    IF Staining of HepG2 using 17622-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GSTT2B antibody (17622-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse lung tissue, human liver tissue, human brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GSTT2B Ready-To-Use IHC Kit. GSTT2B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17622-1-AP targets GSTT2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GSTT2B fusion protein Ag11828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione S-transferase theta 2B (gene/pseudogene)
Synonyms GSTT2, GST class-theta-2, GST class theta 2, Glutathione S-transferase theta-2B, Glutathione S-transferase theta-2
Calculated Molecular Weight 244 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC071700
Gene Symbol GSTT2B
Gene ID (NCBI) 653689
RRIDAB_2116359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CG30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSTT2B antibody 17622-1-APDownload protocol
IF protocol for GSTT2B antibody 17622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Gerontol A Biol Sci Med Sci

PMID: 24285747

Authors - Rojanathammanee Lalida L
View Article
mouseWB

Age (Dordr)

PMID: 25001375

Authors - Lalida Rojanathammanee
View Article
mouseIHC

Sci Signal

Glutathione S-transferases promote proinflammatory astrocyte-microglia communication during brain inflammation.

Authors - Shin-Ichi Kano
  • KD Validated
View Article