验证数据展示
经过测试的应用
Positive IHC detected in | human liver tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
16666-1-AP targets GTDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
免疫原 | GTDC1 fusion protein Ag10145 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glycosyltransferase-like domain containing 1 |
别名 | QTMAN, Mat-Xa, mat Xa, Mannosyltransferase-like protein Xa, Glycosyltransferase-like domain-containing protein 1 |
计算分子量 | 292 aa, 34 kDa, 53 kDa |
GenBank蛋白编号 | BC061699 |
基因名称 | GTDC1 |
Gene ID (NCBI) | 79712 |
RRID | AB_1851177 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q4AE62 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTDC1 (glycosyltransferase-like domain containing 1) encodes a putative glycosyltransferase, whose expression is particularly enriched in the nervous system. GTDC1 glycosylation finely tunes a myriad of neural functions, such as neurite outgrowth, axon guidance, synaptogenesis, membrane excitability, neurotransmission, and neuroinflammation (PMID: 38605125).
实验方案
Product Specific Protocols | |
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IHC protocol for GTDC1 antibody 16666-1-AP | Download protocol |
IF protocol for GTDC1 antibody 16666-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |