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Species Reactivity
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验证数据展示

  • IHC staining of mouse testis using 16666-1-AP

    IHC staining of mouse testis using 16666-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16666-1-AP (GTDC1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 16666-1-AP

    IF Staining of HepG2 using 16666-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GTDC1 antibody (16666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human liver using 16666-1-AP

    IHC staining of human liver using 16666-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16666-1-AP (GTDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • IHC staining of human testis using 16666-1-AP

    IHC staining of human testis using 16666-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16666-1-AP (GTDC1 antibody) at dilution of 1:100 (under 40x lens).

GTDC1 Polyclonal antibody

GTDC1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 16666-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA and More (1)

Synonyms

QTMAN, Mat-Xa, mat Xa, Mannosyltransferase-like protein Xa, Glycosyltransferase-like domain-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16666-1-AP targets GTDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Immunogen GTDC1 fusion protein Ag10145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycosyltransferase-like domain containing 1
Synonyms QTMAN, Mat-Xa, mat Xa, Mannosyltransferase-like protein Xa, Glycosyltransferase-like domain-containing protein 1
Calculated Molecular Weight 292 aa, 34 kDa, 53 kDa
GenBank Accession NumberBC061699
Gene Symbol GTDC1
Gene ID (NCBI) 79712
RRIDAB_1851177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4AE62
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GTDC1 (glycosyltransferase-like domain containing 1) encodes a putative glycosyltransferase, whose expression is particularly enriched in the nervous system. GTDC1 glycosylation finely tunes a myriad of neural functions, such as neurite outgrowth, axon guidance, synaptogenesis, membrane excitability, neurotransmission, and neuroinflammation (PMID: 38605125).

实验方案

Product Specific Protocols
IHC protocol for GTDC1 antibody 16666-1-APDownload protocol
IF protocol for GTDC1 antibody 16666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Rep

A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling.

Authors - Eric Kenney
View Article