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验证数据展示

  • WB analysis of rat testis using 21381-1-AP

    WB analysis of rat testis using 21381-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 21381-1-AP (GTF2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21381-1-AP

    IHC staining of human testis using 21381-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21381-1-AP (GTF2A1 Antibody) at dilution of 1:200 (under 40x lens).

GTF2A1 Polyclonal antibody

GTF2A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21381-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, BGC-823 cells, SKOV-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21381-1-AP targets GTF2A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GTF2A1 fusion protein Ag15994 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIA, 1, 19/37kDa
别名 GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL
计算分子量 276 aa, 42 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC107156
基因名称 GTF2A1
Gene ID (NCBI) 2957
RRIDAB_2878853
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52655
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2A1 antibody 21381-1-APDownload protocol
IHC protocol for GTF2A1 antibody 21381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols