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验证数据展示

  • WB analysis of rat testis using 21381-1-AP

    WB analysis of rat testis using 21381-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 21381-1-AP (GTF2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21381-1-AP

    IHC staining of human testis using 21381-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21381-1-AP (GTF2A1 Antibody) at dilution of 1:200 (under 40x lens).

GTF2A1 Polyclonal antibody

GTF2A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21381-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, BGC-823 cells, SKOV-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21381-1-AP targets GTF2A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2A1 fusion protein Ag15994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIA, 1, 19/37kDa
Synonyms GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL
Calculated Molecular Weight 276 aa, 42 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC107156
Gene Symbol GTF2A1
Gene ID (NCBI) 2957
RRIDAB_2878853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2A1 antibody 21381-1-APDownload protocol
IHC protocol for GTF2A1 antibody 21381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols