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GTF2A1 Polyclonal antibody

GTF2A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21381-1-AP

Print datasheet

Synonyms

GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL

验证数据展示

  • WB analysis of rat testis using 21381-1-AP

    WB analysis of rat testis using 21381-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 21381-1-AP (GTF2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21381-1-AP

    IHC staining of human testis using 21381-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21381-1-AP (GTF2A1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inrat testis tissue, BGC-823 cells, SKOV-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21381-1-AP is GTF2A1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2A1 fusion protein Ag15994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIA, 1, 19/37kDa
Synonyms GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL
Calculated Molecular Weight 276 aa, 42 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC107156
Gene Symbol GTF2A1
Gene ID (NCBI) 2957
RRIDAB_2878853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2A1 antibody 21381-1-APDownload protocol
IHC protocol for GTF2A1 antibody 21381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols