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GTF2A2 Polyclonal antibody

GTF2A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10540-1-AP

Print datasheet

Synonyms

TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2

验证数据展示

  • WB analysis of mouse testis using 10540-1-AP

    WB analysis of mouse testis using 10540-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10540-1-AP (GTF2A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10540-1-AP

    IHC staining of mouse liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells
Positive IHC detected inmouse liver tissue, mouse heart tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10540-1-AP targets GTF2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2A2 fusion protein Ag0825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIA, 2, 12kDa
Synonyms TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC001919
Gene Symbol GTF2A2
Gene ID (NCBI) 2958
RRIDAB_2114351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52657
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region [PMID:20480367]. TFIIA in a complex with TBP mediates transcriptional activity [PMID: 11030333]. GTF2A2 is one subunit of TFIIA.

实验方案

Product Specific Protocols
WB protocol for GTF2A2 antibody 10540-1-APDownload protocol
IHC protocol for GTF2A2 antibody 10540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols