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验证数据展示

  • WB analysis of mouse testis using 10540-1-AP

    WB analysis of mouse testis using 10540-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10540-1-AP (GTF2A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10540-1-AP

    IHC staining of mouse liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GTF2A2 Polyclonal antibody

GTF2A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10540-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells
Positive IHC detected inmouse liver tissue, mouse heart tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10540-1-AP targets GTF2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GTF2A2 fusion protein Ag0825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIA, 2, 12kDa
别名 TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2
计算分子量 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC001919
基因名称 GTF2A2
Gene ID (NCBI) 2958
RRIDAB_2114351
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52657
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region [PMID:20480367]. TFIIA in a complex with TBP mediates transcriptional activity [PMID: 11030333]. GTF2A2 is one subunit of TFIIA.

实验方案

Product Specific Protocols
WB protocol for GTF2A2 antibody 10540-1-APDownload protocol
IHC protocol for GTF2A2 antibody 10540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols