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GTF2E1 Polyclonal antibody

GTF2E1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24405-1-AP

Print datasheet

Synonyms

FE, GTF2E1, TF2E1, TFIIE A, TFIIE alpha

验证数据展示

  • WB analysis of HEK-293 using 24405-1-AP

    WB analysis of HEK-293 using 24405-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24405-1-AP (GTF2E1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 24405-1-AP

    IHC staining of rat kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of rat kidney using 24405-1-AP

    IHC staining of rat kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of mouse kidney using 24405-1-AP

    IHC staining of mouse kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of mouse kidney using 24405-1-AP

    IHC staining of mouse kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human stomach cancer using 24405-1-AP

    IHC staining of human stomach cancer using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human stomach cancer using 24405-1-AP

    IHC staining of human stomach cancer using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human urothelial carcinoma using 24405-1-AP

    IHC staining of human urothelial carcinoma using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human urothelial carcinoma using 24405-1-AP

    IHC staining of human urothelial carcinoma using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat kidney tissue, mouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTF2E1 Ready-To-Use IHC Kit. GTF2E1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24405-1-AP targets GTF2E1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2E1 fusion protein Ag19794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIE, polypeptide 1, alpha 56kDa
Synonyms FE, GTF2E1, TF2E1, TFIIE A, TFIIE alpha
Calculated Molecular Weight 439 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC156997
Gene Symbol GTF2E1
Gene ID (NCBI) 2960
RRIDAB_2879526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2E1 antibody 24405-1-APDownload protocol
IHC protocol for GTF2E1 antibody 24405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols