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TFIIE Beta Polyclonal antibody

TFIIE Beta Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11596-1-AP

Print datasheet

Synonyms

GTF2E2, TFIIE-beta, TFIIE B, TF2E2

验证数据展示

  • WB analysis of K-562 using 11596-1-AP

    WB analysis of K-562 using 11596-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 11596-1-AP

    IF Staining of A375 using 11596-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using TFIIE Beta antibody (11596-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, A375 cells, Jurkat cells
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11596-1-AP targets TFIIE Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TFIIE Beta fusion protein Ag2136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIE, polypeptide 2, beta 34kDa
Synonyms GTF2E2, TFIIE-beta, TFIIE B, TF2E2
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC030572
Gene Symbol GTF2E2
Gene ID (NCBI) 2961
RRIDAB_2232722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).

实验方案

Product Specific Protocols
WB protocol for TFIIE Beta antibody 11596-1-APDownload protocol
IF protocol for TFIIE Beta antibody 11596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.

Authors - De Cegli Rossella R
View Article
humanWB

Cancer Gene Ther

GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma

Authors - Xiaolong Qiao
View Article