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验证数据展示

  • WB analysis of K-562 using 15139-1-AP

    WB analysis of K-562 using 15139-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15139-1-AP

    IF Staining of HeLa using 15139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GTF2F2 Polyclonal antibody

GTF2F2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15139-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, mouse spleen tissue, PC-3 cells, rat spleen tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GTF2F2 Ready-To-Use IHC Kit. GTF2F2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15139-1-AP targets GTF2F2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GTF2F2 fusion protein Ag7313 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIF, polypeptide 2, 30kDa
别名 ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta
计算分子量 28 kDa
观测分子量 30 kDa
GenBank蛋白编号BC001771
基因名称 GTF2F2
Gene ID (NCBI) 2963
RRIDAB_2114554
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13984
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2F2 antibody 15139-1-APDownload protocol
IF protocol for GTF2F2 antibody 15139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols