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GTF2F2 Polyclonal antibody

GTF2F2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15139-1-AP

Print datasheet

Synonyms

ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta

验证数据展示

  • WB analysis of K-562 using 15139-1-AP

    WB analysis of K-562 using 15139-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15139-1-AP

    IF Staining of HeLa using 15139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, mouse spleen tissue, PC-3 cells, rat spleen tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GTF2F2 Ready-To-Use IHC Kit. GTF2F2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15139-1-AP targets GTF2F2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2F2 fusion protein Ag7313 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIF, polypeptide 2, 30kDa
Synonyms ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001771
Gene Symbol GTF2F2
Gene ID (NCBI) 2963
RRIDAB_2114554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13984
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2F2 antibody 15139-1-APDownload protocol
IF protocol for GTF2F2 antibody 15139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols