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GTF2H4 Polyclonal antibody

GTF2H4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15431-1-AP

Print datasheet

Synonyms

TFIIH, TFB2, BTF2 p52

验证数据展示

  • WB analysis using 15431-1-AP

    WB analysis using 15431-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15431-1-AP (GTF2H4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, mouse ovary tissue, mouse thymus tissue, rat thymus tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15431-1-AP targets GTF2H4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2H4 fusion protein Ag7680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIH, polypeptide 4, 52kDa
Synonyms TFIIH, TFB2, BTF2 p52
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC004935
Gene Symbol GTF2H4
Gene ID (NCBI) 2968
RRIDAB_3669207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92759
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GTF2H4 (General Transcription Factor IIH Subunit 4) ,also named as P52, TFB2, TFIIH, is a component of the TFIIH complex, which plays a crucial role in transcription initiation by RNA polymerase II and is also involved in nucleotide excision repair (NER). The role of GTF2H4 in DNA repair mechanisms makes it a potentially important factor in the development and progression of cancer.

实验方案

Product Specific Protocols
WB protocol for GTF2H4 antibody 15431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols