验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse ovary tissue, mouse thymus tissue, rat thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15431-1-AP targets GTF2H4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | GTF2H4 fusion protein Ag7680 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIH, polypeptide 4, 52kDa |
| 别名 | TFIIH, TFB2, BTF2 p52 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC004935 |
| 基因名称 | GTF2H4 |
| Gene ID (NCBI) | 2968 |
| RRID | AB_3669207 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92759 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTF2H4 (General Transcription Factor IIH Subunit 4) ,also named as P52, TFB2, TFIIH, is a component of the TFIIH complex, which plays a crucial role in transcription initiation by RNA polymerase II and is also involved in nucleotide excision repair (NER). The role of GTF2H4 in DNA repair mechanisms makes it a potentially important factor in the development and progression of cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF2H4 antibody 15431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |