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GTF2IRD1 Polyclonal antibody

GTF2IRD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17052-1-AP

Print datasheet

Synonyms

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12

验证数据展示

  • WB analysis of HeLa using 17052-1-AP

    WB analysis of HeLa using 17052-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17052-1-AP

    IHC staining of human testis using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse liver using 17052-1-AP

    IP experiment of mouse liver using 17052-1-AP

    IP result of anti-GTF2IRD1 (IP:17052-1-AP, 4ug; Detection:17052-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • IF Staining of HeLa using 17052-1-AP

    IF Staining of HeLa using 17052-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue, human spleen tissue, human kidney tissue, human heart tissue, human ovary tissue, human skin tissue, human brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17052-1-AP targets GTF2IRD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GTF2IRD1 fusion protein Ag10718 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTF2I repeat domain containing 1
Synonyms BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12
Calculated Molecular Weight 959 aa, 106 kDa
Observed Molecular Weight 145 kDa, 120 kDa
GenBank Accession NumberBC018136
Gene Symbol GTF2IRD1
Gene ID (NCBI) 9569
RRIDAB_2114956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHL9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IHC protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IF protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IP protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols