验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human testis tissue, human spleen tissue, human kidney tissue, human heart tissue, human ovary tissue, human skin tissue, human brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
17052-1-AP targets GTF2IRD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | GTF2IRD1 fusion protein Ag10718 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GTF2I repeat domain containing 1 |
| 别名 | BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12 |
| 计算分子量 | 959 aa, 106 kDa |
| 观测分子量 | 145 kDa, 120 kDa |
| GenBank蛋白编号 | BC018136 |
| 基因名称 | GTF2IRD1 |
| Gene ID (NCBI) | 9569 |
| RRID | AB_2114956 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHL9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF2IRD1 antibody 17052-1-AP | Download protocol |
| IHC protocol for GTF2IRD1 antibody 17052-1-AP | Download protocol |
| IF protocol for GTF2IRD1 antibody 17052-1-AP | Download protocol |
| IP protocol for GTF2IRD1 antibody 17052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
