验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31679-1-AP targets GTF3C1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | GTF3C1 fusion protein Ag36117 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIIC, polypeptide 1, alpha 220kDa |
| 别名 | DKFZp686A111, GTF3C1, TF3C alpha, TFIIIC, TFIIIC 220 kDa subunit, TFIIIC box B binding subunit, TFIIIC220, TFIIICalpha |
| 观测分子量 | 250 kDa |
| GenBank蛋白编号 | BC137229 |
| 基因名称 | GTF3C1 |
| Gene ID (NCBI) | 2975 |
| RRID | AB_3670073 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q12789 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transcription factor IIIC (TF3C or GTF3C), a component of RNA polymerase III, is a multisubunit complex that binds B box promoter elements. It is involved in the assembly of the transcription complex on the initiation tRNA and is required for the transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs (PMID: 8164661).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF3C1 antibody 31679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |