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GTF3C6 Polyclonal antibody

GTF3C6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26382-1-AP

Print datasheet

Synonyms

TFIIIC35, NPD020, CDA020, C6orf51

验证数据展示

  • WB analysis of HeLa using 26382-1-AP

    WB analysis of HeLa using 26382-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26382-1-AP

    IP experiment of HepG2 using 26382-1-AP

    IP result of anti-GTF3C6 (IP:26382-1-AP, 4ug; Detection:26382-1-AP 1:2000) with HepG2 cells lysate 3600ug.

  • IHC staining of human urothelial carcinoma using 26382-1-AP

    IHC staining of human urothelial carcinoma using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C6 antibody included
  • IHC staining of mouse lung using 26382-1-AP

    IHC staining of mouse lung using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C6 antibody included
  • IHC staining of human lung cancer using 26382-1-AP

    IHC staining of human lung cancer using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C6 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human lung cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTF3C6 Ready-To-Use IHC Kit. GTF3C6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26382-1-AP targets GTF3C6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GTF3C6 fusion protein Ag23895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIIC, polypeptide 6, alpha 35kDa
Synonyms TFIIIC35, NPD020, CDA020, C6orf51
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC011593
Gene Symbol GTF3C6
Gene ID (NCBI) 112495
RRIDAB_2880497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF3C6 antibody 26382-1-APDownload protocol
IHC protocol for GTF3C6 antibody 26382-1-APDownload protocol
IP protocol for GTF3C6 antibody 26382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols