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GTPBP2 Polyclonal antibody

GTPBP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11557-1-AP

Print datasheet

Synonyms

GTP binding protein 2, GTPBP2

验证数据展示

  • WB analysis of human kidney using 11557-1-AP

    WB analysis of human kidney using 11557-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11557-1-AP (GTPBP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 11557-1-AP

    IHC staining of human pancreas using 11557-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 11557-1-AP

    IHC staining of human pancreas using 11557-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of mouse spleen using 11557-1-AP

    IP experiment of mouse spleen using 11557-1-AP

    IP result of anti-GTPBP2 (IP:11557-1-AP, 4ug; Detection:11557-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman kidney tissue, human spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11557-1-AP targets GTPBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GTPBP2 fusion protein Ag2113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTP binding protein 2
Synonyms GTP binding protein 2, GTPBP2
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC028347
Gene Symbol GTPBP2
Gene ID (NCBI) 54676
RRIDAB_2115381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX10
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP2 antibody 11557-1-APDownload protocol
IHC protocol for GTPBP2 antibody 11557-1-APDownload protocol
IP protocol for GTPBP2 antibody 11557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols