GTPBP3 Polyclonal antibody

GTPBP3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, ELISA
Cat No. 10764-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IF-Fro, ELISA

MTGP1, MSS1, Mitochondrial GTP-binding protein 1, GTP-binding protein 3, GTPBG3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse breast cancer
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

10764-1-AP targets GTPBP3 in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-Fro, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 GTPBP3 fusion protein Ag1198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTP binding protein 3 (mitochondrial)
别名 MTGP1, MSS1, Mitochondrial GTP-binding protein 1, GTP-binding protein 3, GTPBG3
计算分子量 52 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC019261
基因名称 GTPBP3
Gene ID (NCBI) 84705
RRIDAB_2115389
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969Y2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP3 antibody 10764-1-APDownload protocol
IHC protocol for GTPBP3 antibody 10764-1-APDownload protocol
IF protocol for GTPBP3 antibody 10764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
humanWB

Mol Biol Rep

Spheroid architecture strongly enhances miR-221/222 expression and promotes oxidative phosphorylation in an ovarian cancer cell line through a mechanism that includes restriction of miR-9 expression

Authors - Avery S Ward
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