验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-Fro detected in | mouse breast cancer |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
产品信息
10764-1-AP targets GTPBP3 in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, IF-Fro, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | GTPBP3 fusion protein Ag1198 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | GTP binding protein 3 (mitochondrial) |
别名 | MTGP1, MSS1, Mitochondrial GTP-binding protein 1, GTP-binding protein 3, GTPBG3 |
计算分子量 | 52 kDa |
观测分子量 | 45-50 kDa |
GenBank蛋白编号 | BC019261 |
基因名称 | GTPBP3 |
Gene ID (NCBI) | 84705 |
RRID | AB_2115389 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q969Y2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for GTPBP3 antibody 10764-1-AP | Download protocol |
IHC protocol for GTPBP3 antibody 10764-1-AP | Download protocol |
IF protocol for GTPBP3 antibody 10764-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Cell Discov m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation. | ||
Mol Biol Rep Spheroid architecture strongly enhances miR-221/222 expression and promotes oxidative phosphorylation in an ovarian cancer cell line through a mechanism that includes restriction of miR-9 expression |