验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, human kidney tissue, HepG2 cells |
| Positive IP detected in | HeLa cells, mouse testis tissue |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13897-1-AP targets GTPBP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, drosophila |
| 免疫原 | GTPBP4 fusion protein Ag4859 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GTP binding protein 4 |
| 别名 | NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4 |
| 计算分子量 | 634 aa, 74 kDa |
| 观测分子量 | 74 kDa |
| GenBank蛋白编号 | BC038975 |
| 基因名称 | GTPBP4 |
| Gene ID (NCBI) | 23560 |
| RRID | AB_2279486 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZE4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTPBP4 antibody 13897-1-AP | Download protocol |
| IF protocol for GTPBP4 antibody 13897-1-AP | Download protocol |
| IP protocol for GTPBP4 antibody 13897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. | ||
Proc Natl Acad Sci U S A A genome-scale protein interaction profile of Drosophila p53 uncovers additional nodes of the human p53 network.
| ||
Biochem Biophys Res Commun Up-regulation of GTPBP4 in colorectal carcinoma is responsible for tumor metastasis.
| ||
Biomed Res Int Determining the Clinical Value and Critical Pathway of GTPBP4 in Lung Adenocarcinoma Using a Bioinformatics Strategy: A Study Based on Datasets from The Cancer Genome Atlas.
| ||
Cell Rep UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer | ||
Nucleic Acids Res Distinct interactomes of ADAR1 nuclear and cytoplasmic protein isoforms and their responses to interferon induction |
