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验证数据展示

  • WB analysis using 20133-1-AP

    WB analysis using 20133-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 20133-1-AP (GTPBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20133-1-AP

    IHC staining of human testis using 20133-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20133-1-AP (GTPBP5 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20133-1-AP

    IHC staining of human testis using 20133-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20133-1-AP (GTPBP5 antibody) at dilution of 1:100 (under 40x lens).

GTPBP5 Polyclonal antibody

GTPBP5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20133-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GTP binding protein 5, GTPBP5, ObgH1, Protein obg homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, Jurkat cells, HEK-293 cells, LNCaP cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20133-1-AP targets GTPBP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 GTPBP5 fusion protein Ag14015 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTP binding protein 5 (putative)
别名 GTP binding protein 5, GTPBP5, ObgH1, Protein obg homolog 1
计算分子量 406 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC036716
基因名称 GTPBP5
Gene ID (NCBI) 26164
RRIDAB_10666316
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4K7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP5 antibody 20133-1-APDownload protocol
IHC protocol for GTPBP5 antibody 20133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
View Article