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GTPBP5 Polyclonal antibody

GTPBP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20133-1-AP

Print datasheet

Synonyms

GTP binding protein 5, GTPBP5, ObgH1, Protein obg homolog 1

验证数据展示

  • WB analysis using 20133-1-AP

    WB analysis using 20133-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 20133-1-AP (GTPBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20133-1-AP

    IHC staining of human testis using 20133-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20133-1-AP (GTPBP5 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20133-1-AP

    IHC staining of human testis using 20133-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20133-1-AP (GTPBP5 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inU2OS cells, Jurkat cells, HEK-293 cells, LNCaP cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20133-1-AP targets GTPBP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GTPBP5 fusion protein Ag14015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTP binding protein 5 (putative)
Synonyms GTP binding protein 5, GTPBP5, ObgH1, Protein obg homolog 1
Calculated Molecular Weight 406 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC036716
Gene Symbol GTPBP5
Gene ID (NCBI) 26164
RRIDAB_10666316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4K7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP5 antibody 20133-1-APDownload protocol
IHC protocol for GTPBP5 antibody 20133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
View Article