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GTPBP5 Polyclonal antibody

GTPBP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20133-1-AP

Print datasheet

Synonyms

GTP binding protein 5, GTPBP5, ObgH1, Protein obg homolog 1

验证数据展示

  • WB analysis using 20133-1-AP

    WB analysis using 20133-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 20133-1-AP (GTPBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20133-1-AP

    IHC staining of human testis using 20133-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20133-1-AP (GTPBP5 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20133-1-AP

    IHC staining of human testis using 20133-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20133-1-AP (GTPBP5 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inU2OS cells, Jurkat cells, HEK-293 cells, LNCaP cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 20133-1-AP is GTPBP5 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GTPBP5 fusion protein Ag14015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTP binding protein 5 (putative)
Synonyms GTP binding protein 5, GTPBP5, ObgH1, Protein obg homolog 1
Calculated Molecular Weight 406 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC036716
Gene Symbol GTPBP5
Gene ID (NCBI) 26164
RRIDAB_10666316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4K7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP5 antibody 20133-1-APDownload protocol
IHC protocol for GTPBP5 antibody 20133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
View Article