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验证数据展示

  • WB analysis of mouse pancreas using 18113-1-AP

    WB analysis of mouse pancreas using 18113-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 18113-1-AP

    IF Staining of HepG2 using 18113-1-AP

    Immunofluorescent analysis of HepG2 cells using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human pancreas cancer using 18113-1-AP

    IHC staining of human pancreas cancer using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 18113-1-AP

    IHC staining of human pancreas cancer using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 18113-1-AP

    IHC staining of human kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 18113-1-AP

    IHC staining of human kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 18113-1-AP

    IHC staining of human kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse small intestine using 18113-1-AP

    IHC staining of mouse small intestine using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 18113-1-AP

    IHC staining of mouse kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 18113-1-AP

    IHC staining of mouse small intestine using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Uroguanylin Polyclonal antibody

Uroguanylin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18113-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GUCA2B, UGN, Guanylate cyclase C-activating peptide 2, Guanylate cyclase activator 2B, GCAP II Uroguanylin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pancreas tissue, mouse colon tissue, mouse small intestine tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human stomach tissue, human colon cancer tissue, mouse small intestine tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18113-1-AP targets Uroguanylin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Uroguanylin fusion protein Ag12589 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate cyclase activator 2B (uroguanylin)
别名 GUCA2B, UGN, Guanylate cyclase C-activating peptide 2, Guanylate cyclase activator 2B, GCAP II Uroguanylin
计算分子量 112 aa, 12 kDa
观测分子量 12-13 kDa
GenBank蛋白编号BC093779
基因名称 Uroguanylin
Gene ID (NCBI) 2981
RRIDAB_10858615
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16661
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uroguanylin, also named as GUCA2B, is a candidate intestinal natriuretic hormone that controls the sodium and water balance between the intestine and the kidneys. It has been reported that the uroguanylin knockout mouse exhibits elevated blood pressure. Therefore, the GUCA2B gene is thought to be a susceptibility gene for essential hypertension (EH).

实验方案

Product Specific Protocols
WB protocol for Uroguanylin antibody 18113-1-APDownload protocol
IHC protocol for Uroguanylin antibody 18113-1-APDownload protocol
IF protocol for Uroguanylin antibody 18113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Front Physiol

Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress.

Authors - Nobuhiko Ebisutani
View Article
humanWB

Hum Gene Ther

Gene Co-Expression and miRNA Regulation: A Path to Early Intervention in Colorectal Cancer

Authors - Jason C Huang
View Article