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GUCY1B3 Polyclonal antibody

GUCY1B3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19011-1-AP

Print datasheet

Synonyms

GCS-beta-1, GCS beta 3, GCS beta 1, GC SB3, GC S beta 1



经过测试的应用

Positive WB detected inhuman placenta tissue, mouse lung tissue, rat lung tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19011-1-AP targets GUCY1B3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GUCY1B3 fusion protein Ag5196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate cyclase 1, soluble, beta 3
Synonyms GCS-beta-1, GCS beta 3, GCS beta 1, GC SB3, GC S beta 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 67-71 kDa
GenBank Accession NumberBC047620
Gene Symbol GUCY1B3
Gene ID (NCBI) 2983
RRIDAB_2115817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GUCY1B3 antibody 19011-1-APDownload protocol
IHC protocol for GUCY1B3 antibody 19011-1-APDownload protocol
IF protocol for GUCY1B3 antibody 19011-1-APDownload protocol
IP protocol for GUCY1B3 antibody 19011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.

Authors - Andrea de la Fuente-Alonso
mouseWB

J Ethnopharmacol

Modified Zhenwu Decoction improved intestinal barrier function of experimental colitis through activation of sGC-mediated cGMP/PKG signaling

Authors - Yiqi Xu
mouseWB,IHC

Clin Sci (Lond)

Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.

Authors - Mei Xue
  • KD Validated
mouseWB,IHC

Sci Rep

Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.

Authors - Catarina Rippe
mouseWB

Cell Tissue Res

Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle.

Authors - Baoyi Zhu
ratWB

World Neurosurg

Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway.

Authors - Shi Hai Lan