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  • Featured Product
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GUK1 Polyclonal antibody

GUK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10755-1-AP

Print datasheet

Synonyms

GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1

验证数据展示

  • WB analysis of HEK-293 using 10755-1-AP

    WB analysis of HEK-293 using 10755-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10755-1-AP

    WB analysis of HeLa using 10755-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10755-1-AP

    WB analysis of HEK-293 using 10755-1-AP

    WB result of GUK1 antibody (10755-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

  • IHC staining of human kidney using 10755-1-AP

    IHC staining of human kidney using 10755-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 10755-1-AP

    IHC staining of human kidney using 10755-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10755-1-AP targets GUK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GUK1 fusion protein Ag1205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate kinase 1
Synonyms GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-27 kDa
GenBank Accession NumberBC009914
Gene Symbol GUK1
Gene ID (NCBI) 2987
RRIDAB_2115968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16774
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GUK1 antibody 10755-1-APDownload protocol
IHC protocol for GUK1 antibody 10755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols