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  • Featured Product
  • KD/KO Validated

GUK1 Monoclonal antibody

GUK1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H5E1

Cat no : 67047-1-Ig

Print datasheet

Synonyms

GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1

验证数据展示

  • IHC staining of human thyroid cancer using 67047-1-Ig

    IHC staining of human thyroid cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 67047-1-Ig

    WB analysis of Jurkat using 67047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67047-1-Ig

    WB analysis of HEK-293 using 67047-1-Ig

    WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

  • IF Staining of human thyroid cancer using 67047-1-Ig

    IF Staining of human thyroid cancer using 67047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human thyroid cancer using 67047-1-Ig

    IF Staining of human thyroid cancer using 67047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue, HeLa cells
Positive IHC detected inhuman thyroid cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman thyroid cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67047-1-Ig targets GUK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen GUK1 fusion protein Ag28805 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanylate kinase 1
Synonyms GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-27 kDa
GenBank Accession NumberBC009914
Gene Symbol GUK1
Gene ID (NCBI) 2987
RRIDAB_2882360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16774
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GUK1 antibody 67047-1-IgDownload protocol
IHC protocol for GUK1 antibody 67047-1-IgDownload protocol
IF protocol for GUK1 antibody 67047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols