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GULP1 Polyclonal antibody

GULP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 19902-1-AP

Print datasheet

Synonyms

PTB domain adapter protein CED-6, Protein GULP, GULP, Cell death protein 6 homolog, CED6

验证数据展示

  • WB analysis using 19902-1-AP

    WB analysis using 19902-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19902-1-AP (GULP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 19902-1-AP

    IHC staining of human stomach cancer using 19902-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19902-1-AP (GULP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19902-1-AP targets GULP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GULP1 fusion protein Ag13829 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GULP, engulfment adaptor PTB domain containing 1
Synonyms PTB domain adapter protein CED-6, Protein GULP, GULP, Cell death protein 6 homolog, CED6
Calculated Molecular Weight 167 aa, 19 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC001103
Gene Symbol GULP1
Gene ID (NCBI) 51454
RRIDAB_10916254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GULP1 antibody 19902-1-APDownload protocol
IHC protocol for GULP1 antibody 19902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

The APC/C E3 ligase subunit ANAPC11 mediates FOXO3 protein degradation to promote cell proliferation and lymph node metastasis in urothelial bladder cancer

Authors - Dong Yan
View Article