验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse liver tissue, HL-60 cells, mouse heart tissue, K-562 cells, rat liver tissue |
| Positive IHC detected in | human colon cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
16332-1-AP targets GUSB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GUSB fusion protein Ag9453 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glucuronidase, beta |
| 别名 | Beta G1, Beta glucuronidase, glucuronidase, beta, GUSB, MPS7 |
| 计算分子量 | 651 aa, 75 kDa |
| 观测分子量 | 78 kDa |
| GenBank蛋白编号 | BC014142 |
| 基因名称 | GUSB |
| Gene ID (NCBI) | 2990 |
| RRID | AB_2279538 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08236 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The GUSB gene encodes beta-glucuronidase, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It is a tetrameric glycoprotein composed of identical subunits. GUSB plays an important role in the degradation of dermatan and keratan sulfates. GUSB has two isoforms with the molecular mass of 75 kDa and 69 kDa, and it always can be detected as 78 kDa, 60 kDa and 18 kDa. The 60 kDa and 18 kDa polypeptides are derived by nicking 78-kDa GUSB subunit at Val159-Gly160 (PMID: 1311180, 9268591).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GUSB antibody 16332-1-AP | Download protocol |
| IHC protocol for GUSB antibody 16332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science The adaptive immune system is a major driver of selection for tumor suppressor gene inactivation.
| ||
J Invest Dermatol Distinct roles for Dectin-1 and Dectin-2 in skin wound healing and neutrophilic inflammatory responses. | ||
PLoS One Changes in retinal pigment epithelium related to cigarette smoke: possible relevance to smoking as a risk factor for age-related macular degeneration. | ||
PLoS One Mitochondrial dysfunction leads to deconjugation of quercetin glucuronides in inflammatory macrophages. | ||
PLoS One C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells.
| ||
Gut Microbes Vancomycin relieves tacrolimus-induced hyperglycemia by eliminating gut bacterial beta-glucuronidase enzyme activity |
