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GYPC Polyclonal antibody

GYPC Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 18147-1-AP

Print datasheet

Synonyms

Glycophorin C, Glycoconnectin, GLPC, GE, CD236R

验证数据展示

  • WB analysis of human blood using 18147-1-AP

    WB analysis of human blood using 18147-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18147-1-AP (GYPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18147-1-AP

    IHC staining of human placenta using 18147-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 18147-1-AP

    IHC staining of human placenta using 18147-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 18147-1-AP

    IF Staining of human placenta using 18147-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human placenta using 18147-1-AP

    IF Staining of human placenta using 18147-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of K-562 using 18147-1-AP

    IF Staining of K-562 using 18147-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GYPC antibody (18147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman blood
Positive IHC detected inhuman placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18147-1-AP targets GYPC in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GYPC fusion protein Ag12825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycophorin C (Gerbich blood group)
Synonyms Glycophorin C, Glycoconnectin, GLPC, GE, CD236R
Calculated Molecular Weight 128 aa, 14 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC106051
Gene Symbol GYPC
Gene ID (NCBI) 2995
RRIDAB_2878508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04921
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for GYPC antibody 18147-1-APDownload protocol
IHC protocol for GYPC antibody 18147-1-APDownload protocol
IF protocol for GYPC antibody 18147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar
View Article