验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
Positive IHC detected in | human liver tissue, human skeletal muscle tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10566-1-AP targets GYS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | GYS1 fusion protein Ag0857 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glycogen synthase 1 (muscle) |
别名 | GS, Glycogen synthase1, glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen |
计算分子量 | 84 kDa |
观测分子量 | 84 kDa |
GenBank蛋白编号 | BC007688 |
基因名称 | GYS1 |
Gene ID (NCBI) | 2997 |
RRID | AB_2116401 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P13807 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for GYS1 antibody 10566-1-AP | Download protocol |
IHC protocol for GYS1 antibody 10566-1-AP | Download protocol |
IF protocol for GYS1 antibody 10566-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Int J Biol Sci PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer | ||
J Invest Dermatol Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.
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Arterioscler Thromb Vasc Biol Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy. | ||
Metabolism Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat | ||
J Ethnopharmacol Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis | ||
Mol Nutr Food Res Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice |