Granzyme A Polyclonal antibody
Granzyme A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11288-1-AP targets Granzyme A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Immunogen | Granzyme A fusion protein Ag1818 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) |
| Synonyms | GrA, GZMA, Fragmentin-1, Fragmentin 1, EC:3.4.21.78 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 27-30 kDa, 50-60 kDa |
| GenBank Accession Number | BC015739 |
| Gene Symbol | Granzyme A |
| Gene ID (NCBI) | 3001 |
| RRID | AB_2114392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12544 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Granzyme A antibody 11288-1-AP | Download protocol |
| IHC protocol for Granzyme A antibody 11288-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Rapamycin and Low-dose IL-2 Mediate an Immunosuppressive Microenvironment to Inhibit Benign Prostatic Hyperplasia | ||
J Proteomics Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection. | ||
Immunopharmacol Immunotoxicol Identification of discrepancy between CTLA4 expression and CTLA4 activation in gastric cancer. | ||
Res Vet Sci Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius. | ||
Dev Comp Immunol Perforin and granzyme A release as novel tool to measure NK cell activation in chickens | ||
Front Immunol PD-L1 targeted peptide demonstrates potent antitumor and immunomodulatory activity in cancer immunotherapy |
