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Granzyme B Polyclonal antibody

Granzyme B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31521-1-AP

Print datasheet

Synonyms

GZMB, CSPB, CSP B, CGL1, CGL 1

验证数据展示

  • WB analysis using 31521-1-AP

    WB analysis using 31521-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31521-1-AP (GZMB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 31521-1-AP

    IHC staining of human lymphoma using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 31521-1-AP

    IHC staining of human lymphoma using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31521-1-AP

    IHC staining of human tonsillitis using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31521-1-AP

    IHC staining of human tonsillitis using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNK-92 cells, U-937 cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31521-1-AP targets Granzyme B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Synonyms GZMB, CSPB, CSP B, CGL1, CGL 1
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC030195
Gene Symbol Granzyme B
Gene ID (NCBI) 3002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP10144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

实验方案

Product Specific Protocols
WB protocol for Granzyme B antibody 31521-1-APDownload protocol
IHC protocol for Granzyme B antibody 31521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols