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验证数据展示

  • WB analysis using 17228-1-AP

    WB analysis using 17228-1-AP

    NK-92 cell lysates were subjected to SDS PAGE followed by western blot with 17228-1-AP (GZMH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Granzyme H Polyclonal antibody

Granzyme H Polyclonal Antibody for WB, ELISA
Cat No. 17228-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Cathepsin G like 2, CCP X, CGL 2, CGL2, CSP C, CTLA1, CTSGL2, Cytotoxic serine protease C, Granzyme H, GZMH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNK-92 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17228-1-AP targets Granzyme H in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Granzyme H fusion protein Ag11069 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 granzyme H (cathepsin G-like 2, protein h-CCPX)
别名 Cathepsin G like 2, CCP X, CGL 2, CGL2, CSP C, CTLA1, CTSGL2, Cytotoxic serine protease C, Granzyme H, GZMH
计算分子量 246 aa, 27 kDa
观测分子量~30 kDa
GenBank蛋白编号BC027974
基因名称 Granzyme H
Gene ID (NCBI) 2999
RRIDAB_3085518
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20718
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granzyme H (GzmH) belongs to a family of 5 human serine proteases that are expressed by cytotoxic immune effector cells. It's regarded as an orphan granzyme with unknown biologic functions in immune defense cells. It's reported that this serine protease is predominantly expressed at high levels in natural killer (NK) cells and has chymotrypsin-like (chymase) activity. The calculated molecular weight is 27 kDa, 17228-1-AP can detect a band around 30 kDa. (PMID: 17409270)

实验方案

Product Specific Protocols
WB protocol for Granzyme H antibody 17228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols