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Granzyme K Polyclonal antibody

Granzyme K Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 27894-1-AP

Print datasheet

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

验证数据展示

  • IHC staining of human tonsillitis using 27894-1-AP

    IHC staining of human tonsillitis using 27894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27894-1-AP

    IHC staining of human tonsillitis using 27894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27894-1-AP

    IHC staining of human tonsillitis using 27894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NK-92 using 27894-1-AP

    IF Staining of NK-92 using 27894-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using Granzyme K antibody (27894-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNK-92 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

27894-1-AP targets Granzyme K in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen Granzyme K fusion protein Ag27281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name granzyme K (granzyme 3; NK tryptase II)
Synonyms Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC035802
Gene Symbol GZMK
Gene ID (NCBI) 3003
RRIDAB_2881008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49863
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.

实验方案

Product Specific Protocols
IHC protocol for Granzyme K antibody 27894-1-APDownload protocol
IF protocol for Granzyme K antibody 27894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

iScience

CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response

Authors - Maolin Mu
View Article