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  • Featured Product
  • KD/KO Validated

Mouse Galc Polyclonal antibody, PBS Only

Galc Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11991-1-PBS

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Synonyms

GALCERase, Galactosylceramide beta-galactosidase, Galactocerebroside beta-galactosidase, Galactocerebrosidase, Gacy

验证数据展示

  • WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11991-1-AP (same clone as 11991-1-PBS)

    IHC staining of human gliomas using 11991-1-AP (same clone as 11991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11991-1-AP (Galc antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 11991-1-AP (same clone as 11991-1-PBS)

    IP experiment of NIH/3T3 using 11991-1-AP (same clone as 11991-1-PBS)

    IP result of anti-Galc (IP:11991-1-AP, 4ug; Detection:11991-1-AP 1:300) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11991-1-AP (same clone as 11991-1-PBS)

    IF Staining of mouse brain using 11991-1-AP (same clone as 11991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Galc antibody (11991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

产品信息

11991-1-PBS targets Galc in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Galc fusion protein Ag3914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name galactosylceramidase
Synonyms GALCERase, Galactosylceramide beta-galactosidase, Galactocerebroside beta-galactosidase, Galactocerebrosidase, Gacy
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 80 kDa, 30 kDa, 50 kDa
GenBank Accession NumberBC086671
Gene Symbol Galc
Gene ID (NCBI) 14420
RRIDAB_10641987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54818
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).