• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11991-1-AP (same clone as 11991-1-PBS)

    IHC staining of human gliomas using 11991-1-AP (same clone as 11991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11991-1-AP (Galc antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 11991-1-AP (same clone as 11991-1-PBS)

    IP experiment of NIH/3T3 using 11991-1-AP (same clone as 11991-1-PBS)

    IP result of anti-Galc (IP:11991-1-AP, 4ug; Detection:11991-1-AP 1:300) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11991-1-AP (same clone as 11991-1-PBS)

    IF Staining of mouse brain using 11991-1-AP (same clone as 11991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Galc antibody (11991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Mouse Galc Polyclonal antibody, PBS Only

Galc Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 11991-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

GALCERase, Galactosylceramide beta-galactosidase, Galactocerebroside beta-galactosidase, Galactocerebrosidase, Gacy

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

11991-1-PBS targets Galc in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Galc fusion protein Ag3914 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 galactosylceramidase
别名 GALCERase, Galactosylceramide beta-galactosidase, Galactocerebroside beta-galactosidase, Galactocerebrosidase, Gacy
计算分子量 77 kDa
观测分子量 80 kDa, 30 kDa, 50 kDa
GenBank蛋白编号BC086671
基因名称 Galc
Gene ID (NCBI) 14420
RRIDAB_10641987
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54818
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).