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验证数据展示

  • WB analysis of A375 using 51049-2-AP

    WB analysis of A375 using 51049-2-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 51049-2-AP (Galc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 51049-2-AP

    IHC staining of human prostate cancer using 51049-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51049-2-AP (Galc antibody) at dilution of 1:50 (under 10x lens).

Galc Polyclonal antibody

Galc Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51049-2-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, human brain tissue, human placenta tissue, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51049-2-AP targets Galc in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Galc fusion protein Ag0684 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name galactosylceramidase
Synonyms 2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC086671
Gene Symbol Galc
Gene ID (NCBI) 14420
RRIDAB_670391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54818
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALC, also named as Galactosylceramidase , belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is an enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. (PMID:8634707 )

实验方案

Product Specific Protocols
WB protocol for Galc antibody 51049-2-APDownload protocol
IHC protocol for Galc antibody 51049-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols