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  • Featured Product
  • KD/KO Validated

Galectin-1 Monoclonal antibody, PBS Only

Galectin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G10D2

Cat no : 60223-1-PBS

Print datasheet

Synonyms

Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein

验证数据展示

  • WB analysis using 60223-1-Ig (same clone as 60223-1-PBS)

    WB analysis using 60223-1-Ig (same clone as 60223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60223-1-Ig (same clone as 60223-1-PBS)

    WB analysis of HepG2 using 60223-1-Ig (same clone as 60223-1-PBS)

    WB result of Galectin-1 antibody (60223-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    IF Staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGALS1 antibody included
  • IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGALS1 antibody included
  • IF Staining of U2OS using 60223-1-Ig (same clone as 60223-1-PBS)

    IF Staining of U2OS using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.

产品信息

60223-1-PBS targets Galectin-1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Galectin-1 fusion protein Ag2432 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 1
Synonyms Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC020675
Gene Symbol Galectin-1
Gene ID (NCBI) 3956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09382
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).