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验证数据展示

  • WB analysis of HeLa cells using 14979-1-AP (same clone as 14979-1-PBS)

    WB analysis of HeLa cells using 14979-1-AP (same clone as 14979-1-PBS)

    WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • WB analysis using 14979-1-AP (same clone as 14979-1-PBS)

    WB analysis using 14979-1-AP (same clone as 14979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

    IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Galectin-3 antibody included
  • IP experiment of MCF-7 using 14979-1-AP (same clone as 14979-1-PBS)

    IP experiment of MCF-7 using 14979-1-AP (same clone as 14979-1-PBS)

    IP result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

    IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Galectin-3 antibody included
  • FC experiment of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

    FC experiment of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Galectin-3 Polyclonal antibody (14979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

    IF Staining of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Galectin-3 antibody (14979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Galectin-3 Polyclonal antibody, PBS Only

Galectin-3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 14979-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

GAL3, Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Galectin-3 Ready-To-Use IHC Kit. Galectin-3 primary antibody included.

产品信息

14979-1-PBS targets Galectin-3 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Galectin-3 fusion protein Ag6891 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 3
别名 GAL3, Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35
计算分子量 26 kDa
观测分子量 31 kDa
GenBank蛋白编号BC001120
基因名称 Galectin-3
Gene ID (NCBI) 3958
RRIDAB_2136768
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17931
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).