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验证数据展示

  • WB analysis using 30450-1-AP

    WB analysis using 30450-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30450-1-AP (Galectin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30450-1-AP

    WB analysis of HeLa using 30450-1-AP

    WB result of Galectin 3 antibody (30450-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells.

  • IHC staining of human thyroid cancer using 30450-1-AP

    IHC staining of human thyroid cancer using 30450-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Galectin-3 antibody included
  • IHC staining of human thyroid cancer using 30450-1-AP

    IHC staining of human thyroid cancer using 30450-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Galectin-3 antibody included
  • Featured Product
  • KD/KO验证

Galectin 3 Polyclonal antibody

Galectin 3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30450-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Galectin-3, GAL3, Gal 3, CBP35, CBP 35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman thyroid cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Galectin-3 Ready-To-Use IHC Kit. Galectin-3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30450-1-AP targets Galectin 3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 3
别名 Galectin-3, GAL3, Gal 3, CBP35, CBP 35
计算分子量 26 kDa
观测分子量 29-31 kDa
GenBank蛋白编号BC001120
基因名称 Galectin-3
Gene ID (NCBI) 3958
RRIDAB_3086318
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17931
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

实验方案

Product Specific Protocols
WB protocol for Galectin 3 antibody 30450-1-APDownload protocol
IHC protocol for Galectin 3 antibody 30450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols