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  • Featured Product
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Galectin-3 Recombinant antibody

Galectin-3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4M10

Cat no : 82024-1-RR

Print datasheet

Synonyms

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 4M10

验证数据展示

  • WB analysis using 82024-1-RR

    WB analysis using 82024-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82024-1-RR (Galectin 3 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82024-1-RR

    WB analysis of HeLa using 82024-1-RR

    WB result of Galectin 3 antibody (82024-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells.

  • IP experiment of MCF-7 using 82024-1-RR

    IP experiment of MCF-7 using 82024-1-RR

    IP result of anti-Galectin 3 (IP:82024-1-RR, 4ug; Detection:82024-1-RR 1:10000) with MCF-7 cells lysate 1880 ug.

  • IHC staining of human thyroid cancer using 82024-1-RR

    IHC staining of human thyroid cancer using 82024-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82024-1-RR (Galectin 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Galectin-3 antibody included
  • IF Staining of HeLa using 82024-1-RR

    IF Staining of HeLa using 82024-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin 3 antibody (82024-1-RR, Clone: 4M10 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82024-1-RR

    Affinity and Kinetic Characterization of 82024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82024-1-RR against Human Galectin 3 were performed. The affinity constant is 1.17 nM.

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, A549 cells, A431 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Galectin-3 Ready-To-Use IHC Kit. Galectin-3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82024-1-RR targets Galectin-3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Galectin-3 fusion protein Ag6891 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lectin, galactoside-binding, soluble, 3
Synonyms Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 4M10
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC001120
Gene Symbol Galectin-3
Gene ID (NCBI) 3958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17931
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

实验方案

Product Specific Protocols
WB protocol for Galectin-3 antibody 82024-1-RRDownload protocol
IHC protocol for Galectin-3 antibody 82024-1-RRDownload protocol
IF protocol for Galectin-3 antibody 82024-1-RRDownload protocol
IP protocol for Galectin-3 antibody 82024-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

JCI Insight

HEY1-NCOA2 expression modulates chondrogenic differentiation and induces mesenchymal chondrosarcoma in mice

Authors - Miwa Tanaka
View Article
mouseWB,IHC,IF

Parasit Vectors

Galectin-receptor interaction: a key player in liver fibrosis induced by Schistosoma japonicum infection

Authors - Ziyun Huang
View Article