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Galectin-4 Monoclonal antibody, PBS Only

Galectin-4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat no : 66686-1-PBS

Print datasheet

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

验证数据展示

  • WB analysis of HT-29 using 66686-1-Ig (same clone as 66686-1-PBS)

    WB analysis of HT-29 using 66686-1-Ig (same clone as 66686-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.

产品信息

66686-1-PBS targets Galectin-4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Galectin-4 fusion protein Ag4049 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 4
Synonyms Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4
Calculated Molecular Weight 323 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC034750
Gene Symbol Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2882040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56470
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.